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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two further splice variants were claimed but specific nucleotide sequence information and facts is not yet offered.
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Blocking peptides are peptides that bind exclusively towards the target antibody and block antibody binding. These peptide normally has the epitope regarded from the antibody.
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This information outlines even further possible optimization of mobile and immunoassay parameters to make sure the absolute get more info best benefits are...
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This guide outlines further more possible optimization of cellular and immunoassay parameters to ensure the very best effects are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the target protein. By evaluating the staining in the blocked antibody versus the antibody by yourself, one can see which staining is particular.
Focus on Facts Cyclin E belongs into the highly conserved cyclin family members, whose members are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which lead towards the temporal coordination of every mitotic function. Cyclin E types a posh with and features being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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